UGT1A亚型和组织特异性表达

Abstract UDP-glucuronosyltransferases 1A isoforms belong to a superfamily of microsomal enzymes responsible for glucuronidation of numerous endogenous and exogenous compounds. The nine functional UGT1A isoforms are encoded by a single UGT1A gene locus with multiple first exons. The expression of the UGT1A transcripts was measured by quantitative RT-PCR in 23 normal human tissues. The tissue-specific expression patterns were observed in 13 tissues. To understand the regulation mechanism that is responsible for the tissue-specific expression patterns, we scanned the DNA sequence alignments of the putative promoter regions, exon 1 sequences and intron 1 sequences for those expression-pattern-linked nucleotides. Using one of the expression-pattern-linked nucleotides for livers as an example, we showed that a database comprised of these expression-pattern-linked nucleotides could be used to generate focused hypotheses on the problem of tissue-specific expression,

exon1s
expression-linked nucleotides
ugt1a_exon1_aln.fasta.txt
ugt_exon1.fasta.txt
intron1s
expression-linked nucleotides
ugt1a_intron1.fasta.txt
ugt1a_intron1_aln.fasta.txt
promopter regions
expression-linked nucleotides
ugt1a_promoter.fasta.txt
ugt1a_promoter_aln.fasta.txt