PacBio sequencers produced two types of characteristic reads: CCS (short and low error rate) and CLR (long and high error rate), both of which could be useful for de novo assembly of genomes. PBSIM simulates those PacBio reads by using either a model-based or sampling-based simulation.
If you feel PBSIM is useful in your research, please cite the following paper:
PBSIM: PacBio reads simulator - toward accurate genome assembly
Yukiteru Ono, Kiyoshi Asai and Michiaki Hamada
Bioinformatics (2013) 29 (1) 119-121.
doi: 10.1093/bioinformatics/bts649
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