资 源 简 介
CAPRG SUMMARY
“Contigs Assembly Pipeline using Reference Genome” (CAPRG) assemble long sequence reads for non-model organisms by leveraging a reference genome of a closely-related phylogenetic relative. With the advent of NGS, the assembling of millions of reads is computationally expensive. Various clustering method like megablast, cd-hit have been employed to reduce the memory and processing time. CAPRG utilizes mapping the raw reads of genome/transcriptome against a reference genome and then assembling them to longer contigs that can be utilized for "gene-hunting".
The Pipeline consist of three step:
1. Map the transcriptome raw sequences against the reference genome
2. Store the mapped reads in RDBMS database
3. Assemble reads based on its chromosomal position
