flowregressionmodel

A regression model approach to enable cell morphology correction in high-throughput flow cytometry Cells exposed to stimuli exhibit a wide range of responses ensuring phenotypic variability across the population. Such single cell behavior is often examined by flow cytometry; however, gating procedures typically employed to select a small subpopulation of cells with similar morphological characteristics make it difficult, even impossible, to quantitatively compare cells across a large variety of experimental conditions because these conditions can lead to profound morphological variations. To overcome these limitations, we developed a regression approach to correct for variability in fluorescence intensity due to differences in cell size and granularity without discarding any of the cells, which gating ipso facto does. This approach enables quantitative studies of cellular heterogeneity and transcriptional noise in high-throughput experiments involving thousands of sample

YPR174C-replicate_3-oleate_12h.fcs
YPR174C-replicate_3-oleate_10h.fcs
YPR174C-replicate_3-oleate_8h.fcs
YPR174C-replicate_3-oleate_6h.fcs
YPR174C-replicate_3-oleate_4h.fcs
YPR174C-replicate_3-oleate_2h.fcs
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YPR174C-replicate_3-glucose_10h.fcs
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YPR174C-replicate_3-glucose_4h.fcs
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YPR174C-replicate_2-oleate_12h.fcs
YPR174C-replicate_2-oleate_10h.fcs
YPR174C-replicate_2-oleate_8h.fcs
YPR174C-replicate_2-oleate_6h.fcs
YPR174C-replicate_2-oleate_4h.fcs
YPR174C-replicate_2-oleate_2h.fcs
YPR174C-replicate_2-oleate_0h.fcs
YPR174C-replicate_2-glucose_12h.fcs
YPR174C-replicate_2-glucose_10h.fcs
YPR174C-replicate_2-glucose_6h.fcs
YPR174C-replicate_2-glucose_4h.fcs
YPR174C-replicate_2-glucose_2h.fcs
YPR174C-replicate_1-oleate_12h.fcs
YPR174C-replicate_1-oleate_10h.fcs
YPR174C-replicate_1-oleate_8h.fcs
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YPR174C-replicate_1-oleate_2h.fcs
YPR174C-replicate_1-oleate_0h.fcs
YPR174C-replicate_1-glucose_12h.fcs
YPR174C-replicate_1-glucose_10h.fcs
YPR174C-replicate_1-glucose_8h.fcs
YPR174C-replicate_1-glucose_6h.fcs
YPR174C-replicate_1-glucose_4h.fcs
YPR174C-replicate_1-glucose_2h.fcs
yng1-replicate_3-oleate_12h.fcs
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yng1-replicate_1-oleate_12h.fcs
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yng1-replicate_1-oleate_6h.fcs
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YDL089W-replicate_3-oleate_12h.fcs
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YDL089W-replicate_2-glucose_6h.fcs
YDL089W-replicate_2-glucose_4h.fcs
YDL089W-replicate_2-glucose_2h.fcs
YDL089W-replicate_1-oleate_12h.fcs
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YDL089W-replicate_1-oleate_8h.fcs
YDL089W-replicate_1-oleate_6h.fcs
YDL089W-replicate_1-oleate_4h.fcs
YDL089W-replicate_1-oleate_2h.fcs
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yaf9-replicate_3-oleate_12h.fcs
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yaf9-replicate_2-oleate_12h.fcs
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yaf9-replicate_1-glucose_2h.fcs
WT-POT1-GFP6-replicate_3-oleate_12h.fcs
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WT-POT1-GFP6-replicate_3-glucose_6h.fcs
WT-POT1-GFP6-replicate_3-glucose_4h.fcs
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WT-POT1-GFP5-replicate_3-oleate_12h.fcs
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WT-POT1-GFP4-replicate_3-oleate_12h.fcs
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WT-POT1-GFP3-replicate_2-oleate_12h.fcs
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WT-POT1-GFP1-replicate_3-oleate_12h.fcs
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WT-POT1-GFP1-replicate_2-oleate_12h.fcs
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WT-POT1-GFP1-replicate_1-oleate_4h.fcs
WT-POT1-GFP1-replicate_1-oleate_2h.fcs
WT-POT1-GFP1-replicate_1-oleate_0h.fcs
WT-POT1-GFP1-replicate_1-glucose_12h.fcs
WT-POT1-GFP1-replicate_1-glucose_10h.fcs
WT-POT1-GFP1-replicate_1-glucose_8h.fcs
WT-POT1-GFP1-replicate_1-glucose_6h.fcs
WT-POT1-GFP1-replicate_1-glucose_4h.fcs
WT-POT1-GFP1-replicate_1-glucose_2h.fcs
vps75-replicate_3-oleate_12h.fcs
vps75-replicate_3-oleate_10h.fcs
vps75-replicate_3-oleate_8h.fcs
vps75-replicate_3-oleate_6h.fcs
vps75-replicate_3-oleate_4h.fcs
vps75-replicate_3-oleate_2h.fcs
vps75-replicate_3-oleate_0h.fcs
vps75-replicate_3-glucose_10h.fcs
vps75-replicate_3-glucose_8h.fcs
vps75-replicate_3-glucose_6h.fcs
vps75-replicate_3-glucose_4h.fcs
vps75-replicate_3-glucose_2h.fcs
vps75-replicate_2-oleate_12h.fcs
vps75-replicate_2-oleate_10h.fcs
vps75-replicate_2-oleate_8h.fcs
vps75-replicate_2-oleate_4h.fcs
vps75-replicate_2-oleate_2h.fcs
vps75-replicate_2-oleate_0h.fcs
vps75-replicate_2-glucose_12h.fcs
vps75-replicate_2-glucose_10h.fcs
vps75-replicate_2-glucose_8h.fcs
vps75-replicate_2-glucose_6h.fcs
vps75-replicate_2-glucose_4h.fcs
vps75-replicate_2-glucose_2h.fcs
vps75-replicate_1-oleate_12h.fcs
vps75-replicate_1-oleate_10h.fcs
vps75-replicate_1-oleate_8h.fcs
vps75-replicate_1-oleate_6h.fcs
vps75-replicate_1-oleate_2h.fcs
vps75-replicate_1-oleate_0h.fcs
vps75-replicate_1-glucose_12h.fcs
vps75-replicate_1-glucose_10h.fcs
vps75-replicate_1-glucose_8h.fcs
vps75-replicate_1-glucose_6h.fcs
vps75-replicate_1-glucose_4h.fcs
vps75-replicate_1-glucose_2h.fcs
vps72-replicate_3-oleate_12h.fcs
vps72-replicate_3-oleate_10h.fcs
vps72-replicate_3-oleate_8h.fcs
vps72-replicate_3-oleate_6h.fcs
vps72-replicate_3-oleate_4h.fcs
vps72-replicate_3-oleate_2h.fcs
vps72-replicate_3-oleate_0h.fcs
vps72-replicate_3-glucose_10h.fcs
vps72-replicate_3-glucose_8h.fcs
vps72-replicate_3-glucose_6h.fcs
vps72-replicate_3-glucose_4h.fcs
vps72-replicate_3-glucose_2h.fcs
vps72-replicate_2-oleate_12h.fcs
vps72-replicate_2-oleate_10h.fcs
vps72-replicate_2-oleate_8h.fcs
vps72-replicate_2-oleate_6h.fcs
vps72-replicate_2-oleate_4h.fcs
vps72-replicate_2-oleate_2h.fcs
vps72-replicate_2-oleate_0h.fcs
vps72-replicate_2-glucose_12h.fcs
vps72-replicate_2-glucose_10h.fcs
vps72-replicate_2-glucose_8h.fcs
vps72-replicate_2-glucose_6h.fcs
vps72-replicate_2-glucose_4h.fcs
vps72-replicate_2-glucose_2h.fcs
vps72-replicate_1-oleate_12h.fcs
vps72-replicate_1-oleate_10h.fcs
vps72-replicate_1-oleate_8h.fcs
vps72-replicate_1-oleate_6h.fcs
vps72-replicate_1-oleate_4h.fcs
vps72-replicate_1-oleate_2h.fcs
vps72-replicate_1-oleate_0h.fcs
vps72-replicate_1-glucose_12h.fcs
vps72-replicate_1-glucose_10h.fcs
vps72-replicate_1-glucose_8h.fcs
vps72-replicate_1-glucose_6h.fcs
vps72-replicate_1-glucose_4h.fcs
vps72-replicate_1-glucose_2h.fcs
vps71-replicate_3-oleate_12h.fcs
vps71-replicate_3-oleate_10h.fcs
vps71-replicate_3-oleate_8h.fcs
vps71-replicate_3-oleate_6h.fcs
vps71-replicate_3-oleate_4h.fcs
vps71-replicate_3-oleate_2h.fcs
vps71-replicate_3-oleate_0h.fcs
vps71-replicate_3-glucose_10h.fcs
vps71-replicate_3-glucose_8h.fcs
vps71-replicate_3-glucose_6h.fcs
vps71-replicate_3-glucose_4h.fcs
vps71-replicate_3-glucose_2h.fcs
vps71-replicate_2-oleate_12h.fcs
vps71-replicate_2-oleate_10h.fcs
vps71-replicate_2-oleate_8h.fcs
vps71-replicate_2-oleate_6h.fcs
vps71-replicate_2-oleate_4h.fcs
vps71-replicate_2-oleate_2h.fcs
vps71-replicate_2-oleate_0h.fcs
vps71-replicate_2-glucose_12h.fcs
vps71-replicate_2-glucose_10h.fcs
vps71-replicate_2-glucose_8h.fcs
vps71-replicate_2-glucose_6h.fcs
vps71-replicate_2-glucose_4h.fcs
vps71-replicate_2-glucose_2h.fcs
vps71-replicate_1-oleate_12h.fcs
vps71-replicate_1-oleate_10h.fcs
vps71-replicate_1-oleate_8h.fcs
vps71-replicate_1-oleate_6h.fcs
vps71-replicate_1-oleate_4h.fcs
vps71-replicate_1-oleate_2h.fcs
vps71-replicate_1-oleate_0h.fcs
vps71-replicate_1-glucose_12h.fcs
vps71-replicate_1-glucose_10h.fcs
vps71-replicate_1-glucose_8h.fcs
vps71-replicate_1-glucose_6h.fcs
vps71-replicate_1-glucose_4h.fcs
vps71-replicate_1-glucose_2h.fcs
uip3-replicate_3-oleate_12h.fcs
uip3-replicate_3-oleate_10h.fcs
uip3-replicate_3-oleate_8h.fcs
uip3-replicate_3-oleate_6h.fcs
uip3-replicate_3-oleate_4h.fcs
uip3-replicate_3-oleate_2h.fcs
uip3-replicate_3-oleate_0h.fcs
uip3-replicate_3-glucose_10h.fcs
uip3-replicate_3-glucose_8h.fcs
uip3-replicate_3-glucose_6h.fcs
uip3-replicate_3-glucose_4h.fcs
uip3-replicate_3-glucose_2h.fcs
uip3-replicate_2-oleate_12h.fcs
uip3-replicate_2-oleate_10h.fcs
uip3-replicate_2-oleate_8h.fcs
uip3-replicate_2-oleate_6h.fcs
uip3-replicate_2-oleate_4h.fcs
uip3-replicate_2-oleate_2h.fcs
uip3-replicate_2-oleate_0h.fcs
uip3-replicate_2-glucose_12h.fcs
uip3-replicate_2-glucose_10h.fcs
uip3-replicate_2-glucose_8h.fcs
uip3-replicate_2-glucose_6h.fcs
uip3-replicate_2-glucose_4h.fcs
uip3-replicate_2-glucose_2h.fcs
uip3-replicate_1-oleate_12h.fcs
uip3-replicate_1-oleate_10h.fcs
uip3-replicate_1-oleate_8h.fcs
uip3-replicate_1-oleate_6h.fcs
uip3-replicate_1-oleate_2h.fcs
uip3-replicate_1-oleate_0h.fcs
uip3-replicate_1-glucose_12h.fcs
uip3-replicate_1-glucose_10h.fcs
uip3-replicate_1-glucose_8h.fcs
uip3-replicate_1-glucose_6h.fcs
uip3-replicate_1-glucose_4h.fcs
uip3-replicate_1-glucose_2h.fcs
ubp8-replicate_3-oleate_12h.fcs
ubp8-replicate_3-oleate_10h.fcs
ubp8-replicate_3-oleate_8h.fcs
ubp8-replicate_3-oleate_6h.fcs
ubp8-replicate_3-oleate_4h.fcs
ubp8-replicate_3-oleate_2h.fcs
ubp8-replicate_3-oleate_0h.fcs
ubp8-replicate_3-glucose_10h.fcs
ubp8-replicate_3-glucose_8h.fcs
ubp8-replicate_3-glucose_6h.fcs
ubp8-replicate_3-glucose_4h.fcs
ubp8-replicate_3-glucose_2h.fcs
ubp8-replicate_2-oleate_12h.fcs
ubp8-replicate_2-oleate_10h.fcs
ubp8-replicate_2-oleate_8h.fcs
ubp8-replicate_2-oleate_4h.fcs
ubp8-replicate_2-oleate_2h.fcs
ubp8-replicate_2-oleate_0h.fcs
ubp8-replicate_2-glucose_12h.fcs
ubp8-replicate_2-glucose_10h.fcs
ubp8-replicate_2-glucose_8h.fcs
ubp8-replicate_2-glucose_6h.fcs
ubp8-replicate_2-glucose_4h.fcs
ubp8-replicate_2-glucose_2h.fcs
ubp8-replicate_1-oleate_12h.fcs
ubp8-replicate_1-oleate_10h.fcs
ubp8-replicate_1-oleate_8h.fcs
ubp8-replicate_1-oleate_6h.fcs
ubp8-replicate_1-oleate_2h.fcs
ubp8-replicate_1-oleate_0h.fcs
ubp8-replicate_1-glucose_12h.fcs
ubp8-replicate_1-glucose_10h.fcs
ubp8-replicate_1-glucose_8h.fcs
ubp8-replicate_1-glucose_6h.fcs
ubp8-replicate_1-glucose_4h.fcs
ubp8-replicate_1-glucose_2h.fcs
taf14-replicate_3-oleate_12h.fcs
taf14-replicate_3-oleate_10h.fcs
taf14-replicate_3-oleate_8h.fcs
taf14-replicate_3-oleate_6h.fcs
taf14-replicate_3-oleate_4h.fcs
taf14-replicate_3-oleate_2h.fcs
taf14-replicate_3-oleate_0h.fcs
taf14-replicate_3-glucose_10h.fcs
taf14-replicate_3-glucose_8h.fcs
taf14-replicate_3-glucose_6h.fcs
taf14-replicate_3-glucose_4h.fcs
taf14-replicate_3-glucose_2h.fcs
taf14-replicate_2-oleate_12h.fcs
taf14-replicate_2-oleate_10h.fcs
taf14-replicate_2-oleate_8h.fcs
taf14-replicate_2-oleate_4h.fcs
taf14-replicate_2-oleate_2h.fcs
taf14-replicate_2-oleate_0h.fcs
taf14-replicate_2-glucose_12h.fcs
taf14-replicate_2-glucose_10h.fcs
taf14-replicate_2-glucose_8h.fcs
taf14-replicate_2-glucose_6h.fcs
taf14-replicate_2-glucose_4h.fcs
taf14-replicate_2-glucose_2h.fcs
taf14-replicate_1-oleate_12h.fcs
taf14-replicate_1-oleate_10h.fcs
taf14-replicate_1-oleate_8h.fcs
taf14-replicate_1-oleate_6h.fcs
taf14-replicate_1-oleate_2h.fcs
taf14-replicate_1-oleate_0h.fcs
taf14-replicate_1-glucose_12h.fcs
taf14-replicate_1-glucose_10h.fcs
taf14-replicate_1-glucose_8h.fcs
taf14-replicate_1-glucose_6h.fcs
taf14-replicate_1-glucose_4h.fcs
taf14-replicate_1-glucose_2h.fcs