easy-dhpsf

This package of MATLAB routines with accompanying GUI allows scientific users to measure the 3D location of single molecules when using the double-helix point spread function (DH-PSF) widefield microscope. Features * Double-Gaussian estimator is calibrated via an axial scan of bright immobile fluorescent emitters (e.g. beads). * Template-matching and double-helix recognition algorithm is calibrated via setting an image correlation threshold against single-molecule data. * Tiff stacks of SM images are analyzed using template matching followed by double-Gaussian fitting to extract estimates of the molecule positions. * The motion/drift of fiduciaries (e.g. fluorescent beads) during an experiment can tracked and removed form SM localization data. * 3D localization data can be exported to .csv format for post-processing and visualization. * Basic 3D scatterplot and 2D z-projection histogram visualizations are included. | Contents

easy_dhpsf.m
f_calDHPSF.m
f_calSMidentification.m
f_concatSMfits.m
f_debugMoleculeFits.m
f_doubleGaussianVector.m
f_doubleGaussianVectorJacobian.m
f_fitSMs.m
f_hist2.m
f_iconRead.m
f_scatter3.m
f_trackFiducials.m
f_waveletBackground.m
f_waveletFidTracks.m